![]() You might get some different colors due to variation in pH, but other than that, you're ok. The ladder will be separated fine, and it will transfer fine - so if all you're using it for is to tell when your gel has run far enough and whether transfer has occurred sufficiently, any pre-stained ladder will be fine. The gels were stained after the transfer process to examine the amount of. The protein ladder is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. This only comes into play if you're using the standards to find the molecular weight of your protein. To directly compare the efficiency of HMW protein transfer using rapid semi-dry and wet transfer methods, western blot detection of three HMW protein targets, EGFR (190 kDa), mTOR (289 kDa), and Ecm29 (205 kDa), was performed using these methods in parallel (Figure 4). Thermo Scientific PageRuler Plus Prestained Protein Ladder is a mixture of nine blue-, orange-, and green-stained proteins (10 to 250 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. ![]() However, as I said, the migration of the pre-stained standards is sensitive to pH, so you'd need to calibrate your pre-stained standard with a non-stained standard to get the apparent molecular weights as they behave in your system. There's no reason why any ladder would not work with this gel system. This gel uses chloride as the mobile anion other gel chemistries use other anions. Note: The WesternSure Ladder is not recommended for use when stripping and re-probing Western blots. ![]() It usually has a stacking gel of pH 6.8 and a separating gel of pH 8.8. The WesternSure chemiluminescent protein ladder is the only pre-stained, multi-colored protein ladder for both film and digital chemiluminescence detection. A Tris-HCL gel is just the original Laemmli gel, around since the 1970's.
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